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Slide #1. Genetics and Genomics of Drosophila Mating Behavior Slide #2. Mating Behaviors are Complex Traits Slide #3. Quantitative Genetics of Mating Behavior: Slide #4. Flies R Us Slide #5. Power of Drosophila Quantitative Genetics Slide #6. Mating Behavior in Drosophila Slide #7. What Genes Affect Drosophila Mating Behavior? Slide #8. What Genes Affect Drosophila Mating Behavior? Slide #9. What Genes Affect Variation in Slide #10. What Genes Affect Variation in Slide #11. Drosophila simulans and D. mauritiana Slide #12. D. simulans ? ? D. mauritiana ? <BR> ? <BR> F1 ? ? D. mauritiana ? <BR> ? <BR>3 Populations of 1000 <BR>Single Recombinant Backcross Flies (BC) Slide #13. 32 markers <BR> <BR> 19 SNPs <BR> 13 Microsatellites Slide #14. Trait <BR> <BR>mate (yes/no) Slide #15. CIM examines if a QTL lies within an interval between <BR>two markers <BR> H0 : There is not a QTL between two markers <BR> H1 : There is a QTL between two markers <BR> <BR> Likelihood Ratio (LR) = -2ln(H0 / H1) <BR> <BR> Multiple regressions control for the effects of the other QTLs <BR> 1000 permutations to get the significance threshold Slide #16. QTLs in D. mauritiana Females for Discrimination Against D. simulans Males Slide #17. Trait <BR> <BR>mate (yes/no) <BR> Slide #18. QTLs in D. simulans Males that D. mauritiana Females Discriminate Against Slide #19. Traits Measured <BR> <BR>copulation duration Slide #20. QTLs in D. mauritiana Males that D. simulans Females Discriminate Against Slide #21. Do the Same QTLs Affect Reproductive Isolation in Males and Females? Slide #22. Do the Same QTL Affect Reproductive Isolation in the Two Species? Slide #23. Few QTLs, moderate – large effects <BR> <BR> Additive gene action <BR> <BR> QTLs for female choice are different than those for the male traits they are choosing against <BR> <BR> D. mauritiana females appear to have a different mode of mate choice from D. simulans females <BR> <BR> High resolution recombination mapping necessary to identify individual genes Slide #24. What Genes Affect Variation in Slide #25. Oregon/2b Slide #26. roo Element Markers Slide #27. Mating Behavior Assay Slide #28. Variation Among RI Lines Slide #29. 72A–85F Slide #30. Genome Scan Mapping Results Slide #31. Deficiency Mapping Slide #32. Deficiency Complementation Mapping Slide #33. Deficiency Complementation Mapping Slide #34. Deficiency Complementation Mapping Slide #35. Mutant Complementation Test Slide #36. Candidate Genes Slide #37. Candidate Genes Slide #38. Candidate Genes Slide #39. QTLs Affecting Mating Behavior in Slide #40. What Genes Affect Variation in Slide #41. Artificial Selection for Mating Speed Slide #42. Response to Selection for Mating Speed Slide #43. Heritability of Mating Speed Slide #44. Slow Females are Picky Slide #45. No Difference in Courtship Latency Slide #46. Fast Males Court More Vigorously Slide #47. Affymetrix Drosophila GeneChip 2.0 Slide #48. Fast 1 Fast 2 Slow 1 Slow 2 Females • • • • • • • • • • • • <BR>Males • • • • • • • • • • • • Slide #49. Transcriptional Response to Selection Slide #50. Chromosome Distribution of Significant Probe Sets Slide #51. Biological Process GO Categories Slide #52. Genes Previously Implicated Slide #53. Interesting Novel Candidate Genes: Slide #54. Interesting Novel Candidate Genes: Slide #55. Interesting Novel Candidate Genes: Slide #56. Interesting Novel Candidate Genes: Slide #57. Divergent Selection for Mating Speed Slide #58. Collaborators